MSE PRO Rat MTL(Motilin) ELISA Kit

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SKU: EK0064328-48T
Regular price $ 319.95

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MSE PRO™ Rat MTL(Motilin) ELISA Kit

Specifications:

Product name Rat MTL(Motilin) ELISA Kit
Reactivity Rat
Alternative Names MLN; MAP; Promotilin; Motilin-associated peptide
Assay Type Competitive Inhibition
Sensitivity 29.75 pg/mL
Standard 6000 pg/mL
Detection Range 93.75-6000 pg/mL
Sample type Serum, plasma and other biological fluids
Assay length 2h
Research Area Endocrinology; Gastroenterology; Hormone metabolism
Test principle This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat MTL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MTL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MTL in the samples is then determined by comparing the OD of the samples to the standard curve.