MSE PRO Pig LPS(Lipopolysaccharide) ELISA Kit
SKU: EK0064750-96T
MSE PRO™ Pig LPS(Lipopolysaccharide) ELISA Kit
Specifications:
| Product name | Pig LPS(Lipopolysaccharide) ELISA Kit |
| Reactivity | Pig |
| Alternative Names | LOS; Lipoglycans; Lipooligosaccharide; Lipo-Oligosaccharide; Endotoxin |
| Assay Type | Sandwich |
| Sensitivity | 0.047 ng/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Signal transduction |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig LPS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig LPS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig LPS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig LPS in the samples is then determined by comparing the OD of the samples to the standard curve. |