MSE PRO Pig βEP(Beta-Endorphin) ELISA Kit
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MSE PRO™ Pig βEP(Beta-Endorphin) ELISA Kit
Specifications:
| Product name | Pig βEP(Beta-Endorphin) ELISA Kit |
| Reactivity | Pig |
| Alternative Names | Beta EP; b-EP |
| Assay Type | Competitive Inhibition |
| Sensitivity | 4.95 pg/mL |
| Standard | 1000 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Type | Serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 2.5h |
| Research Area | Signal transduction; Endocrinology; Neuro science; Hormone metabolism |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig βEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig βEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig βEP in the samples is then determined by comparing the OD of the samples to the standard curve. |