MSE PRO NE(Noradrenaline) ELISA Kit
SKU: EK0064257-48T
MSE PRO™ NE (Noradrenaline) ELISA Kit
Specifications:
| Product name | NE (Noradrenaline) ELISA Kit |
| Reactivity | General |
| Alternative Names | NA; NAd; Norepinephrine; Norepi |
| Assay Type | Competitive Inhibition |
| Sensitivity | 26 pg/mL |
| Standard | 5000 pg/mL |
| Detection Range | 78.13-5000 pg/mL |
| Sample type | serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| Assay length | 2h |
| Research Area | Signal transduction; Infection immunity; Neuro science; Hormone metabolism |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NE in the samples is then determined by comparing the OD of the samples to the standard curve. |