MSE PRO Mouse TRAb(Thyroid Stimulating Hormone Receptor Antibody) ELISA Kit
SKU: EK0064888-96T
MSE PRO™ Mouse TRAb(Thyroid Stimulating Hormone Receptor Antibody) ELISA Kit
Specifications:
| Product name | Mouse TRAb(Thyroid Stimulating Hormone Receptor Antibody) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | TSHRAb |
| Assay Type | Sandwich |
| Sensitivity | 0.63 ng/mL |
| Standard | 100 ng/mL |
| Detection Range | 1.57-100 ng/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Signal Transduction; Neuroscience |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TRAb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TRAb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TRAb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TRAb in the samples is then determined by comparing the OD of the samples to the standard curve. |