MSE PRO Mouse NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
SKU: EK0064326-48T
MSE PRO™ Mouse NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
Specifications:
| Product name | Mouse NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb |
| Assay Type | Sandwich |
| Sensitivity | 28 pg/mL |
| Standard | 5000 pg/mL |
| Detection Range | 78.13-5000 pg/mL |
| Sample type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay length | 3.5h |
| Research Area | Endocrinology; Cardiovascular biology |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve. |