MSE PRO Mouse LAB7-2(B-Lymphocyte Activation Antigen B7-2) ELISA Kit
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MSE PRO™ Mouse LAB7-2(B-Lymphocyte Activation Antigen B7-2) ELISA Kit
Specifications:
| Product name | Mouse LAB7-2(B-Lymphocyte Activation Antigen B7-2) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | CD86; B7-2; B70; FUN-1; CD28LG2; CD28 Antigen Ligand 2,B7-2 Antigen; CTLA-4 counter-receptor B7.2 |
| Assay Type | Sandwich |
| Sensitivity | 0.048 ng/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Signal transduction; CD & Adhesion molecule; Tumor immunity; Infection immunity; Immune molecule |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LAB7-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LAB7-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LAB7-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LAB7-2 in the samples is then determined by comparing the OD of the samples to the standard curve. |