MSE PRO Mouse GC(Glucagon) ELISA Kit
SKU: EK0064330-48T
MSE PRO™ Mouse GC (Glucagon) ELISA Kit
Specifications:
| Product name | Mouse GC (Glucagon) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | GCG; GLP1; GLP2; GRPP; Glicentin-Related Polypeptide; Glucagen; Oxyntomodulin; Incretin hormone |
| Assay Type | Competitive Inhibition |
| Sensitivity | 7.2 pg/mL |
| Standard | 1500 pg/mL |
| Detection Range | 23.44-1500 pg/mL |
| Sample type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay length | 2h |
| Research Area | Endocrinology |
| Uniprot ID | P55095 |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse GC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GC in the samples is then determined by comparing the OD of the samples to the standard curve. |