MSE PRO Mouse E2(Estradiol) ELISA Kit
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MSE PRO™ Mouse E2(Estradiol) ELISA Kit
Specifications:
| Product name | Mouse E2(Estradiol) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | 17B-Estradiol; Oestradiol; Beta-Estradiol |
| Assay Type | Competitive Inhibition |
| Sensitivity | 14.45 pg/mL |
| Standard | 3000 pg/mL |
| Detection Range | 46.88-3000 pg/mL |
| Sample Type | serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 2.5h |
| Research Area | Endocrinology; Reproductive science; Hormone metabolism |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse E2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse E2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse E2 in the samples is then determined by comparing the OD of the samples to the standard curve. |