MSE PRO Mouse cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit
SKU: EK0064284-48T
MSE PRO™ Mouse cTnT/TNNT2 (Troponin T Type 2, Cardiac) ELISA Kit
Specifications:
| Product name | Mouse cTnT/TNNT2 (Troponin T Type 2, Cardiac) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | CMH2; cTnT; TnTc; Cardiomyopathy, Hypertrophic 2; Cardiac muscle troponin T; TNNT2; Troponin T Type 2, Cardiac |
| Assay Type | Sandwich |
| Sensitivity | 5.5 pg/mL |
| Standard | 1000 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay length | 3.5h |
| Research Area | Cardiovascular biology |
| Uniprot ID | P50752 |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse cTnT/TNNT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse cTnT/TNNT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse cTnT/TNNT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse cTnT/TNNT2 in the samples is then determined by comparing the OD of the samples to the standard curve. |