MSE PRO Mouse CHI3L1(Chitinase-3-Like Protein 1) ELISA Kit
Regular price
$ 319.95
Couldn't load pickup availability
Still have questions? Ask our experts!
MSE PRO™ Mouse CHI3L1(Chitinase-3-Like Protein 1) ELISA Kit
Specifications:
| Product name | Mouse CHI3L1(Chitinase-3-Like Protein 1) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | YKL-40; Chondrocyte protein 40 |
| Assay Type | Sandwich |
| Sensitivity | 0.16 ng/mL |
| Standard | 50 ng/mL |
| Detection Range | 0.78-50 ng/mL |
| Sample Type | serum, plasma, saliva, tears and other biological fluids and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Metabolic pathway |
| Uniprot ID | Q61362 |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CHI3L1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CHI3L1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CHI3L1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CHI3L1 in the samples is then determined by comparing the OD of the samples to the standard curve. |