MSE PRO Mouse Anti-KLH IgM(Keyhole Limpet Hemocyanin IgM) ELISA Kit
SKU: EK0064572-96T
MSE PRO™ Mouse Anti-KLH IgM(Keyhole Limpet Hemocyanin IgM) ELISA Kit
Specifications:
| Product name | Mouse Anti-KLH IgM(Keyhole Limpet Hemocyanin IgM) ELISA Kit |
| Reactivity | Mouse |
| Alternative Names | Keyhole limpet IgM |
| Assay Type | Sandwich |
| Sensitivity | 0.51 ng/mL |
| Standard | 100 ng/mL |
| Detection Range | 1.57-100 ng/mL |
| Sample Type | Serum, plasma, tissue homogenates |
| Assay Length | 3.5h |
| Research Area | Immunology |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Anti-KLH IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Anti-KLH IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Anti-KLH IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Anti-KLH IgM in the samples is then determined by comparing the OD of the samples to the standard curve. |