MSE PRO Human PSG11(Pregnancy-Specific Beta-1-Glycoprotein 11) ELISA Kit

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SKU: EK0064458-96T
Regular price $ 319.95

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MSE PRO™ Human PSG11(Pregnancy-Specific Beta-1-Glycoprotein 11) ELISA Kit

Specifications:

Product name Human PSG11(Pregnancy-Specific Beta-1-Glycoprotein 11) ELISA Kit
Reactivity Human
Alternative Names PSBG-11; PSBG-13; PSG13; PSG14
Assay Type Sandwich
Sensitivity 0.18 ng/mL
Standard 50 ng/mL
Detection Range 0.78-50 ng/mL
Sample Type serum, plasma, saliva, tears and other biological fluids and other biological fluids
Assay Length 3.5h
Research Area Reproductive science
Uniprot ID Q9UQ72
Test principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSG11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PSG11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PSG11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSG11 in the samples is then determined by comparing the OD of the samples to the standard curve.