MSE PRO Human MAP4K4(Mitogen-activated Protein Kinase 4) ELISA Kit
SKU: EK0064663-96T
MSE PRO™ Human MAP4K4(Mitogen-activated Protein Kinase 4) ELISA Kit
Specifications:
| Product name | Human MAP4K4(Mitogen-activated Protein Kinase 4) ELISA Kit |
| Reactivity | Human |
| Alternative Names | HPK/GCK-like kinase HGK; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; Nck-interacting kinase |
| Assay Type | Sandwich |
| Sensitivity | 12.4 pg/mL |
| Standard | 3000 pg/mL |
| Detection Range | 46.88-3000 pg/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Enzyme & Kinase |
| Uniprot ID | O95819 |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MAP4K4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MAP4K4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MAP4K4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MAP4K4 in the samples is then determined by comparing the OD of the samples to the standard curve. |