MSE PRO Human GP2(Glycoprotein 2, Zymogen Granule Membrane) ELISA Kit
SKU: EK0064883-96T
MSE PRO™ Human GP2(Glycoprotein 2, Zymogen Granule Membrane) ELISA Kit
Specifications:
| Product name | Human GP2(Glycoprotein 2, Zymogen Granule Membrane) ELISA Kit |
| Reactivity | Human |
| Alternative Names | ZAP75; Pancreatic Secretory Granule Membrane Major Glycoprotein GP2; Pancreatic zymogen granule membrane protein GP-2 |
| Assay Type | Sandwich |
| Sensitivity | 0.17 ng/mL |
| Standard | 20 ng/mL |
| Detection Range | 0.32-20 ng/mL |
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids. |
| Assay Length | 3.5h |
| Research Area | Endocrinology |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GP2 in the samples is then determined by comparing the OD of the samples to the standard curve. |