MSE PRO Human GLP1(Glucagon Like Peptide 1) ELISA Kit
SKU: EK0064327-48T
MSE PRO™ Human GLP1 (Glucagon Like Peptide 1) ELISA Kit
Specifications:
| Product name | Human GLP1 (Glucagon Like Peptide 1) ELISA Kit |
| Reactivity | Human |
| Alternative Names | Glp1 |
| Assay Type | Competitive Inhibition |
| Sensitivity | 4.36 pg/mL |
| Standard | 1000 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample type | serum, plasma and other biological fluids |
| Assay length | 2h |
| Research Area | Endocrinology; Cardiovascular biology; Hepatology; Hormone metabolism |
| Test principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GLP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GLP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GLP1 in the samples is then determined by comparing the OD of the samples to the standard curve. |