MSE PRO Human GATA4(GATA Binding Protein 4) ELISA Kit
SKU: EK0064854-96T
MSE PRO™ Human GATA4(GATA Binding Protein 4) ELISA Kit
Specifications:
| Product name | Human GATA4(GATA Binding Protein 4) ELISA Kit |
| Reactivity | Human |
| Alternative Names | Transcription factor GATA-4 |
| Assay Type | Sandwich |
| Sensitivity | 0.7 ng/mL |
| Standard | 100 ng/mL |
| Detection Range | 1.56-100 ng/mL |
| Sample Type | Tissue homogenates, cell lysates and other biological fluids. |
| Assay Length | 3.5h |
| Research Area | Signal transduction; Developmental science |
| Uniprot ID | P43694 |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GATA4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GATA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GATA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GATA4 in the samples is then determined by comparing the OD of the samples to the standard curve. |