MSE PRO Human CPA1(Carboxypeptidase A1) ELISA Kit
SKU: EK0064614-96T
MSE PRO™ Human CPA1(Carboxypeptidase A1) ELISA Kit
Specifications:
| Product name | Human CPA1(Carboxypeptidase A1) ELISA Kit |
| Reactivity | Human |
| Alternative Names | CPA |
| Assay Type | Sandwich |
| Sensitivity | 0.89 ng/mL |
| Standard | 150 ng/mL |
| Detection Range | 2.35-150 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Enzyme & Kinase |
| Uniprot ID | P15085 |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CPA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CPA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CPA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CPA1 in the samples is then determined by comparing the OD of the samples to the standard curve. |