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MSE PRO Human CALHM6(Protein FAM26F) ELISA Kit

SKU: EK0064870-96T

  • $ 45795


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MSE PRO™ Human CALHM6(Protein FAM26F) ELISA Kit

Specifications:

Product name Human CALHM6(Protein FAM26F) ELISA Kit
Reactivity Human
Alternative Names C6orf187; FAM26F
Assay Type Sandwich
Sensitivity 0.059 ng/mL
Standard 10 ng/mL
Detection Range 0.16-10 ng/mL
Sample Type Serum, plasma, tissue homogenates and other biological fluids
Assay Length 3.5h
Research Area Signal transduction
Uniprot ID Q5R3K3
Test principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CALHM6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CALHM6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CALHM6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CALHM6 in the samples is then determined by comparing the OD of the samples to the standard curve.