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MSE PRO Human C1orf77(Chromatin target of PRMT1 protein) ELISA Kit

SKU: EK0064496-96T

  • $ 31995


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MSE PRO™ Human C1orf77(Chromatin target of PRMT1 protein) ELISA Kit

Specifications:

Product name Human C1orf77(Chromatin target of PRMT1 protein) ELISA Kit
Reactivity Human
Alternative Names Chromosome 1 Open Reading Frame 77; CHTOP; pp7704; SRAG; FOP; FL-SRAG; C10orf77; C1orf77; SRAG-5; SRAG-3;
Assay Type Sandwich
Sensitivity 0.059 ng/mL
Standard 10 ng/mL
Detection Range 0.16-10 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Assay Length 3.5h
Research Area Signal transduction
Uniprot ID Q9Y3Y2
Test principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C1orf77. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C1orf77. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C1orf77, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C1orf77 in the samples is then determined by comparing the OD of the samples to the standard curve.