MSE PRO Hamster IP10(Interferon Gamma Induced Protein 10kDa) ELISA Kit
SKU: EK0064511-96T
MSE PRO™ Hamster IP10(Interferon Gamma Induced Protein 10kDa) ELISA Kit
Specifications:
| Product name | Hamster IP10(Interferon Gamma Induced Protein 10kDa) ELISA Kit |
| Reactivity | Hamster |
| Alternative Names | CXCL10; C7; IFI10; INP10; SCYB10; Crg2; GIP10; Mob1; Chemokine C-X-C Motif Ligand 10; Small Inducible Cytokine Subfamily B(Cys-X-Cys)Member 10 |
| Assay Type | Sandwich |
| Sensitivity | 5.8 pg/mL |
| Standard | 1000 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Cytokine; Tumor immunity; Infection immunity |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hamster IP10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hamster IP10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hamster IP10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hamster IP10 in the samples is then determined by comparing the OD of the samples to the standard curve. |