MSE PRO Fast and Precise Real‑Time PCR System

MSE PRO™ Fast and Precise Real‑Time PCR System

Description

MA0066615 is 96-well Real-Time Quantitative PCR system. Based on innovative signal gathering and spectrum separation technology, together with unique temperature control technology, it reached an advanced level in terms of detection sensitivity, multi-color crosstalk correction, temperature uniformity and accuracy. The instrument is available with a 4-channel and is compatible with a wide spectrum of qPCR dye-based and probe-based chemistry, easily realizing multiplex PCR applications. The instrument supports standard analysis methods like absolute quantification, genotype, and gene expressing analysis.

Features

Fast and Precise Thermal Cycling

High-efficiency gold-plated Peltier provides heating and cooling rate up to 6°C/s completing 45 cycle amplification within 30 minutes; Innovative alloy block design provides high temperature uniformity of ±0.2°C. The auxiliary heating plate effectively reduces the edge effect.

Innovative Optic Design

Based on confocal scanning fluorescence detection technology, it can effectively remove the interference of background light and excitation light,obtain fluorescent signals with a high signal-to-noise ratio, and save the use of fluorescent probes and dyes. High-performance, long-life single-color LEDs are used as excitation light sources, without thermal attenuation, and maintenance-free for life. High-performance silicon PMT can obtain high fluorescence signals under weak excitation light and reduce photobleaching. The instrument uses linear fluorescence time-sharing scanning technology to ensure the uniformity of all fluorescence acquisitions, improving the accuracy and uniformity of CT. Thereby, the edge effects is effectively reduced and ROX calibration is not required.

Excellent Reproducibility 

Robust design ensures superior uniformity from run to run.Amplification curves for 96 replicates shown on a linear plot a logarithmic plot.Average quantification cycle(Cq)=24±0.10.

Up to 10-log dynamic range

Results on the nQ96-X4/5 System show excellent reproducibility and resolution down to very low copy numbers.

Melting Curve Analysis

96 replicates of human genomic DNA were amplified using SYBR reagent. The reactions were run under fast run mode, showing thermal uniformity as measured by the derivative peak with a melting temperature (Tm) of 77.5°C (standard deviation 0.05°C).

High Resolution

The excellent uniformity of temperature together with quick detection of data guarantee the reaction temperature and time to be identical between different tubes, so it can reliably detect 1.33-fold differences in target amount. The figure 1 shows the curve of 6 gradient dilutions: in 2X The figure 2 and 3 shows the curve of 1.5X dilutions.

Specification