MSE PRO EasyStep Rat E2(Estradiol) ELISA Kit
SKU: EK0064683-96T
MSE PRO™ EasyStep Rat E2(Estradiol) ELISA Kit
Specifications:
| Product name | EasyStep Rat E2(Estradiol) ELISA Kit |
| Reactivity | Rat |
| Alternative Names | 17B-Estradiol; Oestradiol; Beta-Estradiol |
| Assay Type | Competitive Inhibition |
| Sensitivity | 25 pg/mL |
| Standard | 1600 pg/mL |
| Detection Range | 25-1600 pg/mL |
| Sample Type | Serum |
| Assay Length | 1.5h |
| Research Area | Endocrinology; Reproductive science; Hormone metabolism |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat E2 antigen, and the Rat E2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat E2 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat E2 in the samples is then determined by comparing the OD of the samples to the standard curve. |