MSE PRO EasyStep Human VCAM1(Vascular Cell Adhesion Molecule 1) ELISA Kit
SKU: EK0064876-96T
MSE PRO™ EasyStep Human VCAM1(Vascular Cell Adhesion Molecule 1) ELISA Kit
Specifications:
| Product name | EasyStep Human VCAM1(Vascular Cell Adhesion Molecule 1) ELISA Kit |
| Reactivity | Human |
| Alternative Names | CD106; INCAM-100; L1CAM |
| Assay Type | Sandwich |
| Sensitivity | 0.87 ng/mL |
| Standard | 80 ng/mL |
| Detection Range | 1.25-80 ng/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | CD & Adhesion molecule; Tumor immunity; Infection immunity |
| Uniprot ID | P19320 |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VCAM1, and the Human VCAM1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VCAM1. After TMB substrate solution is added, only those wells that contain Human VCAM1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VCAM1 in the samples is then determined by comparing the OD of the samples to the standard curve. |