MSE PRO EasyStep Human OC(Osteocalcin) ELISA Kit
SKU: EK0064560-96T
MSE PRO™ EasyStep Human OC(Osteocalcin) ELISA Kit
Specifications:
| Product name | EasyStep Human OC(Osteocalcin) ELISA Kit |
| Reactivity | Human |
| Alternative Names | BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein |
| Assay Type | Sandwich |
| Sensitivity | 0.14 ng/mL |
| Standard | 15 ng/mL |
| Detection Range | 0.23-15 ng/mL |
| Sample Type | Serum |
| Assay Length | 1.5h |
| Research Area | Metabolic pathway; Endocrinology; Hormone metabolism; Bone metabolism |
| Uniprot ID | P02818 |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OC, and the Human OC standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human OC. After TMB substrate solution is added, only those wells that contain Human OC and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OC in the samples is then determined by comparing the OD of the samples to the standard curve. |