MSE PRO EasyStep Human MPO(Myeloperoxidase) ELISA Kit
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MSE PRO™ Easy Step Human MPO(Myeloperoxidase) ELISA Kit
Specifications:
| Product name | Easy Step Human MPO(Myeloperoxidase) ELISA Kit |
| Reactivity | Human |
| Alternative Names | Mpo; Myeloperoxidase; MPO |
| Assay Type | Sandwich |
| Sensitivity | 184 pg/mL |
| Standard | 40 ng/mL |
| Detection Range | 0.63-40 ng/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Enzyme & Kinase; Infection immunity; Cardiovascular biology; Hepatology |
| Uniprot ID | P05164 |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MPO, and the Human MPO standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MPO. After TMB substrate solution is added, only those wells that contain Human MPO and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MPO in the samples is then determined by comparing the OD of the samples to the standard curve. |