MSE PRO EasyStep Human LpPLA2(Phospholipase A2, Lipoprotein Associated) ELISA Kit
SKU: EK0064848-96T
MSE PRO™ EasyStep Human LpPLA2(Phospholipase A2, Lipoprotein Associated) ELISA Kit
Specifications:
| Product name | EasyStep Human LpPLA2(Phospholipase A2, Lipoprotein Associated) ELISA Kit |
| Reactivity | Human |
| Alternative Names | PLA2G7; PAF-AH; PAFAH; Lp-PLA2; LDL-PLA2; Platelet Activating Factor Acetylhydrolase, Plasma; Phospholipase A2,Group VII; LDL-associated phospholipase A2 |
| Assay Type | Sandwich |
| Sensitivity | 0.78 ng/mL |
| Standard | 50 ng/mL |
| Detection Range | 0.78-50 ng/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Enzyme & Kinase; Metabolic pathway; Infection immunity |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LpPLA2, and the Human LpPLA2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human LpPLA2. After TMB substrate solution is added, only those wells that contain Human LpPLA2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LpPLA2 in the samples is then determined by comparing the OD of the samples to the standard curve. |