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MSE PRO EasyStep Human EFNA1(Ephrin A1) ELISA Kit

SKU: EK0064703-96T

  • $ 58795


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MSE PRO™ EasyStep Human EFNA1(Ephrin A1) ELISA Kit

Specifications:

Product name EasyStep Human EFNA1(Ephrin A1) ELISA Kit
Reactivity Human
Alternative Names B61; ECKLG; EFL1; EPLG1; LERK1; TNFAIP4; EPH-related receptor tyrosine kinase ligand 1; Tumor necrosis factor alpha-induced protein 4; Immediate early response protein B61
Assay Type Sandwich
Sensitivity 22.5 pg/mL
Standard 10 ng/mL
Detection Range 0.16-10 ng/mL
Sample Type Serum, plasma
Assay Length 1.5h
Research Area Neuro science
Uniprot ID P20827
Test principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EFNA1, and the Human EFNA1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human EFNA1. After TMB substrate solution is added, only those wells that contain Human EFNA1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EFNA1 in the samples is then determined by comparing the OD of the samples to the standard curve.