MSE PRO EasyStep Human CEA(Carcinoembryonic Antigen) ELISA Kit
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MSE PRO™ EasyStep Human CEA(Carcinoembryonic Antigen) ELISA Kit
Specifications:
| Product name | EasyStep Human CEA(Carcinoembryonic Antigen) ELISA Kit |
| Reactivity | Human |
| Alternative Names | CD66E; CD66; CEACAM5; Carcinoembryonic Antigen-related Cell Adhesion Molecule 5; Carcinoembryonic Antigen |
| Assay Type | Sandwich |
| Sensitivity | 7.5 pg/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | Serum, plasma |
| Assay Length | 1.5h |
| Research Area | Tumor immunity; Gastroenterology |
| Uniprot ID | P06731 |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CEA, and the Human CEA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human CEA. After TMB substrate solution is added, only those wells that contain Human CEA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CEA in the samples is then determined by comparing the OD of the samples to the standard curve. |