MSE PRO Cor(Cortisol) ELISA Kit
SKU: EK0064320-48T
MSE PRO™ Cor (Cortisol) ELISA Kit
Specifications:
| Product name | Cor (Cortisol) ELISA Kit |
| Reactivity | General |
| Alternative Names | Hydrocortisone |
| Assay Type | Competitive Inhibition |
| Sensitivity | 0.93 ng/mL |
| Standard | 200 ng/mL |
| Detection Range | 3.13-200 ng/mL |
| Sample type | serum, plasma, urine and other biological fluids |
| Assay length | 2h |
| Research Area | Metabolic pathway; Endocrinology; Hormone metabolism |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cor. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cor. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cor in the samples is then determined by comparing the OD of the samples to the standard curve. |