MSE PRO cGMP(Cyclic Guanosine Monophosphate) ELISA Kit
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MSE PRO™ cGMP (Cyclic Guanosine Monophosphate) ELISA Kit
Specifications:
| Product name | cGMP (Cyclic Guanosine Monophosphate) ELISA Kit |
| Reactivity | General |
| Alternative Names | c-GMP; 3',5'-Cyclic GMP; Cyclic 3',5'-GMP; Guanosine 3',5'-Cyclic Phosphate |
| Assay Type | Competitive Inhibition |
| Sensitivity | 43.8 pg/mL |
| Standard | 10000 pg/mL |
| Detection Range | 156.25-10000 pg/mL |
| Sample type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay length | 2h |
| Research Area | Signal transduction; Metabolic pathway; |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with cGMP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to cGMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of cGMP in the samples is then determined by comparing the OD of the samples to the standard curve. |