MSE PRO cAMP(Cyclic Adenosine Monophosphate) ELISA Kit
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MSE PRO™ cAMP (Cyclic Adenosine Monophosphate) ELISA Kit
Specifications:
| Product name | cAMP (Cyclic Adenosine Monophosphate) ELISA Kit |
| Reactivity | General |
| Alternative Names | c-AMP; 3'-5'-Cyclic Adenosine Monophosphate; Adenosine Cyclophosphate |
| Assay Type | Competitive Inhibition |
| Sensitivity | 92.2 pg/mL |
| Standard | 20000 pg/mL |
| Detection Range | 312.5-20000 pg/mL |
| Sample type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay length | 2h |
| Research Area | Signal transduction; Metabolic pathway; |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with cAMP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to cAMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of cAMP in the samples is then determined by comparing the OD of the samples to the standard curve. |