MSE PRO 4-HNE(4-Hydroxynonenal) ELISA Kit
SKU: EK0064317-48T
MSE PRO™ 4-HNE (4-Hydroxynonenal) ELISA Kit
Specifications:
| Product name | 4-HNE (4-Hydroxynonenal) ELISA Kit |
| Reactivity | General |
| Alternative Names | 4-HNE; HNE; 4-hydroxy-2-nonenal; 4-Hydroxynonenal |
| Assay Type | Competitive Inhibition |
| Sensitivity | 0.38 ng/mL |
| Standard | 40 ng/mL |
| Detection Range | 0.63-40 ng/mL |
| Sample type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay length | 2h |
| Research Area | Signal transduction; Metabolic pathway ;Infection immunity; Hormone metabolism; Dermatology |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 4-HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve. |