Mitochondrial Extraction Assay Kit
Mitochondrial Extraction Assay Kit
| SKU # | E-BC-E001 |
Product Details
Properties
| Sample type | Animal tissue |
| Other instruments required | High-speed freezing centrifuge, 5mL Glass homogenizer |
| Other reagents required | Double distilled water, PBS(0.01 M，pH=7.4) |
| Storage | -20℃ |
| Valid period | 12 months |
Detection Principle
The principle of mitochondrial separation and purification: after rupturing cells mechanically, the debris and huge organelles are removed by low-speed differential centrifugation, then purified mitochondria will be obtained by high-speed differential centrifugation.
Kit Components & Storage
| Item | Component | Specification | Storage |
| Reagent 1 | Lysis Buffer | 60 mL × 1 vial | -20°C, 12 months |
| Reagent 2 | Mito-Wash Buffer | 30 mL × 1 vial | -20°C, 12 months, shading light |
| Reagent 3 | Store Buffer | 15 mL × 1 vial | -20°C, 12 months |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.