Interscience Blender Bags with Culture Media: instaBAG® Fraser ½ Filter Bag with Dehydrated Media

instaBAG® Fraser ½ is a filter bag which encloses a dehydrated pre-dosed media, allowing a 2-in-1 process: media and sample preparation for microbiological analyses. Fraser ½ is used as a medium for primary selective enrichment for the detection and enumeration of Listeria monocytogenes.

 MAIN FEATURES

• Filter bag with dehydrated pre-dosed media
• Type of dehydrated media: supplemented Fraser ½
• Filter porosity: < 250 micrometers
• Compatible with any laboratory blender

APPLICATIONS

instaBAG® supplemented Fraser ½ is used in the food industry and public institutes research. Fraser allows the suspension of solid samples of 25 g diluted at 1/10th for the detection of Listeria monocytogenes. This selective media is already supplemented. It is validated according to ISO 11133.

TECHNICAL SPECIFICATIONS

Ready-to-use bag with pre-dosed dehydrated media
• Bag with lateral non-woven filter
• For pipetting
• For all types of samples (fibrous, pasty, …)
• Multilayer®: multicoated reinforced complex
• Filter porosity: < 250 micron
• Rigid and transparent
• No contact between the sample and the blender during homogenization of the sample
• Compatible with any 400 mL blender
• Type of dehydrated media: supplemented Fraser ½
• Fraser ½ is used as a medium for primary selective enrichment or as a diluent for the detection and enumeration of Listeria monocytogenes and Listeria spp. (ISO 11290-1, ISO 11290-2) in food and environmental samples.
• Blending time: 2 minutes
• Approved for food contact: Regulation (EC) No 1935/2004
• Gamma ray treated: Gamma 10 to 25 kGy, with certificate
• Shelf life: 30 months
• Storage conditions: room temperature (+ 10°C à + 30°C). Close the pouch after opening to avoid humidity.
• Available in 225 mL size
• In compliance with ISO 7218, ISO 6887 and FDA BAM (Bacteriological Analytical Manual)
• The control of Fraser ½ medium performances follows ISO 11133. A compliance certificate is available on request.
• Designed and made in France

COMPOSITION

Typical formula* for 1L of reconstituted medium:
• Enzymatic digest of animal tissues: 5,0 g
• Casein enzymatic digest: 5,0 g
• Meat extract: 5.0 g
• Yeast extract: 5.0 g
• Sodium chloride: 20.0 g
• Disodium hydrogen phosphate dihydrate: 12.0 g
• Potassium dihydrogen phosphate: 1.35 g
• Esculin: 1.0 g
• Lithium chloride: 3.0 g
• Sodium salt of nalidixic acid: 0.01 g
• Acriflavine hydrochloride: 0.0125 g
• Iron (III) ammoniacal citrate: 0.5 g
* Adjusted to meet the performance criteria required by the standards

HOW TO USE

• Place the sample in the filter bag instaBAG®
• Add water (sterile, deionized, at room temperature)
• Place the bag in a lab blender to dissolve the culture media and homogenize the sample
• Blend for 2 minutes to perfectly dissolve the powder in the water

instaBAG® Fraser ½

Ref. 114 220

•  Bag dimensions: 190 x 300 mm
•  Weight of dehydrated media: 13 g (Fraser ½ at 57.87 g/L)
• Sample weight to analyze: 25 g (± 5%)
• Volume of water to be added (sterile, deionized, at room temperature): 187 mL (± 2%)
• Aluminum pouch of 10 filter bags
• Box dimensions: 34 x 28 x 11 cm
• Weight: 2.25 kg
• Box of 80 filter bags

MSE Supplies is an authorized distributor of Interscience products.