Histone H1 Polyclonal Antibody
SKU: E-AB-31676-200
Histone H1 Polyclonal Antibody
| SKU # | E-AB-31676 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Applications | WB, IHC-p, IF |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC-p, IF |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from human Histone H1 around the non-phosphorylation site of Thr17. |
| Abbre | Histone H1 |
| Synonyms | H1F3, H1F4, H1F5, HIST1H1B, HIST1H1D, HIST1H1E, Histone H1.3, Histone H1.4, Histone H1.5, Histone H1a, Histone H1b, Histone H1c, Histone H1s-2, Histone H1s-3, Histone H1s-4 |
| Swissprot | |
| Calculated MW | 23 kDa |
| Observed MW | 31 28kDa |
| Cellular Localization | Extracellular region or secreted, extracellular exosome, Nucleus, nuclear heterochromatin, nuclear nucleosome, nucleus. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-1:2000 |
| IHC | 1:100-1:300 |
| IF | 1:200-1:1000 |
Background
May play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling.