HIMEDIA RPMI-1640 w/ L-Glutamine, 25mM HEPES buf
SKU: AL060A-500ML
HIMEDIA RPMI-1640 w/ L-Glutamine, 25mM HEPES buffer and Sodium Bicarbonate 500ML
Product Description:
Roswell Park Memorial Institute (RPMI) media are a series of media developed by Moore et al for the culture of human normal and neoplastic cells in vitro. RPMI-1640 is the most commonly used medium in the series. A modification of McCoy's 5A medium, the medium was specifically designed to support the growth of human lymphoblastoid cells in suspension culture. Presently the medium is extensively used for a wide range of anchorage dependant cell lines. The medium needs to be supplemented with 5-20% fetal bovine serum. The medium is also known to support growth of cells in the absence of serum.
AL060A is RPMI-1640 medium supplemented with L-glutamine, 25mM HEPES buffer and sodium bicarbonate. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37°C prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
Composition:
|
Ingredients |
mg/L |
|
INORGANIC SALTS |
|
|
Calcium nitrate tetrahydrate |
100.000 |
|
Disodium hydrogen phosphate anhydrous |
800.000 |
|
Magnesium sulphate anhydrous |
48.840 |
|
Potassium chloride |
400.000 |
|
Sodium bicarbonate |
2000.000 |
|
Sodium chloride |
6000.000 |
|
AMINO ACIDS |
|
|
Glycine |
10.000 |
|
L-Arginine hydrochloride |
241.000 |
|
L-Asparagine monohydrate |
50.000 |
|
L-Aspartic acid |
20.000 |
|
L-Cystine dihydrochloride |
65.150 |
|
L-Glutamic acid |
20.000 |
|
L-Glutamine |
300.000 |
|
L-Histidine hydrochloride monohydrate |
20.960 |
|
L-Hydroxyproline |
20.000 |
|
L-Isoleucine |
50.000 |
|
L-Leucine |
50.000 |
|
L-Lysine hydrochloride |
40.000 |
|
L-Methionine |
15.000 |
|
L-Phenylalanine |
15.000 |
|
L-Proline |
20.000 |
|
L-Serine |
30.000 |
|
L-Threonine (Allo free) |
20.000 |
|
L-Tryptophan |
5.000 |
|
L-Tyrosine sodium salt dihydrate |
28.830 |
|
L-Valine |
20.000 |
|
VITAMINS |
|
|
Choline chloride |
3.000 |
|
D-Biotin |
0.200 |
|
D-Ca-Pantothenate |
0.250 |
|
Folic acid |
1.000 |
|
Niacinamide |
1.000 |
|
Pyridoxine hydrochloride |
1.000 |
|
Riboflavin |
0.200 |
|
Thiamine hydrochloride |
1.000 |
|
Vitamin B12 |
0.005 |
|
i-Inositol |
35.000 |
|
p-Amino benzoic acid (PABA) |
1.000 |
|
OTHERS |
|
|
D-Glucose |
2000.000 |
|
Glutathione reduced |
1.000 |
|
HEPES Buffer |
5958.000 |
|
Phenol red sodium salt |
5.300 |