HIMEDIA Leibovitz’s L-15 Medium w/ L-Glutamine w/o Sodium Pyruvate and Sodium Bicarbonate
SKU: AT164-10X1L
HIMEDIA Leibovitz’s L-15 Medium w/ L-Glutamine w Sodium Pyruvate and Sodium Bicarbonate 10X1L, 5L
Product Description :
Leibovitz's Medium was specifically designed to grow cells in a CO2 free atmosphere. The standard sodium bicarbonate/CO2 buffering system is replaced by combination of free basic amino acids, phosphate buffers and higher levels of galactose and sodium pyruvate. As a result, the medium does not require supplementation with sodium bicarbonate and can be used under conditions of free gaseous exchange with the atmosphere. The medium can be used to grow human tumor cells and embryonic cells and also established cell lines like HeLa and Hep-2. The medium is frequently used in diagnostic virology where tissue cell lines or strains need to be grown in closed systems. Leibovitz's medium obviates the need of frequent medium change.
AT164 is Leibovitz's Medium with L-glutamine. It does not contain sodium pyruvate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
Composition:
|
Ingredients |
mg/L |
|
INORGANIC SALTS |
|
|
Calcium chloride dihydrate |
185.000 |
|
Magnesium chloride hexahydrate |
200.000 |
|
Magnesium sulphate anhydrous |
97.720 |
|
Potassium chloride |
400.000 |
|
Potassium phosphate monobasic |
60.000 |
|
Sodium chloride |
8000.000 |
|
Sodium phosphate dibasic anhydrous |
190.120 |
|
AMINO ACIDS |
|
|
L-Alanine |
225.000 |
|
Glycine |
200.000 |
|
L-Arginine (free base) |
500.000 |
|
L-Asparagine monohydarte |
284.070 |
|
L-Cysteine (free base) |
120.000 |
|
L-Glutamine |
300.000 |
|
L-Histidine (free base) |
250.000 |
|
L-Isoleucine |
125.000 |
|
L-Leucine |
125.000 |
|
L-Lysine hydrochloride |
93.700 |
|
L-Methionine |
75.000 |
|
L-Phenylalanine |
125.000 |
|
L-Serine |
200.000 |
|
L-Threonine |
300.000 |
|
L-Tryptophan |
20.000 |
|
L-Tyrosine disodium salt dihydrate |
430.000 |
|
VITAMINS |
|
|
Choline chloride |
1.000 |
|
D-Ca-Pantothenate |
1.000 |
|
Folic acid |
1.000 |
|
Nicotinamide |
1.000 |
|
Pyridoxine hydrochloride |
1.000 |
|
Riboflavin-5-phosphate sodium salt |
0.100 |
|
Thiamine monophosphate |
1.000 |
|
i-Inositol |
2.000 |
|
OTHERS |
|
|
D-Glucose |
900.000 |
|
Phenol red sodium salt |
10.000 |
Directions :
- Suspend 13.5gms in 900ml tissue culture grade water with constant, gentle stirring until the powder is completely dissolved. Do not heat the water. Note: Presence of slight haziness in the medium is due to inherent nature of some of the ingredients in the composition. However, this will not affect the performance of the medium.
- Adjust the pH to 0.2 - 0.3 pH units below the desired pH using 1N HCl or 1N NaOH since the pH tends to rise during filtration.
- Make up the final volume to 1000ml with tissue culture grade water.
- Sterilize the medium immediately by filtering through a sterile membrane filter with porosity of 0.22 micron or less, using positive pressure rather than vacuum to minimize the loss of carbon dioxide.
- Aseptically add sterile supplements as required and dispense the desired amount of sterile medium into sterile containers.
- Store liquid medium at 2-8ºC and in dark till use.