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Glucose Uptake Fluorometric Assay Kit - MSE Supplies LLC

Glucose Uptake Fluorometric Assay Kit

  • $ 1,70095

Glucose Uptake Fluorometric Assay Kit

SKU # E-BC-F041
Detection Instrument Fluorescence microplate reader (Ex/Em=530 nm/590 nm)
Detection Method Fluorescence method


Product Details

Properties

Sample Type Cells
Sensitivity 0.02 nmol/μL
Detection Range 0.02-0.3 nmol/μL
Detection Method Fluorescence method
Assay Time 100 min
Other Reagents Required KRPH solution (20 mM Hepes, 5 mM KH2PO4, 1 mM MgSO4, 1 mM CaCl2, 136 mM NaCl, 4.7 mM KCl, 2% BSA, pH 7.4)
Storage This product can be stored at -20°C for 12 months with shading light.
Valid period 12 months


Detection Principle

2-DG is up-taken by the cells, converted to 2-DG-6P, which is catalyzed by glucose dehydrogenase to produce 6PDG. Meanwhile, NADP+ is converted to NADPH. The generated NADPH converts the probe into fluorescent substances under the action of myocardial yellow transferase. The glucose uptake can be calculated by measuring the fluorescence intensity at the excitation wavelength of 530 nm and the emission wavelength of 590 nm.

 

Kit Components & Storage

Item Component Size 1 (96 T) Size 2 (500 Assays) Storage
Reagent 1 Acid Reagent 10 mL ×1 vial 55 mL ×1 vial -20℃, 12 months
Reagent 2 Alkali Reagent 10 mL × 1 vial 55 mL × 1 vial -20℃, 12 months
Reagent 3 Chromogenic Agent 25 mL × 1 vial 45 mL × 3 vials -20℃, 12 months shading light
Reagent 4 Enzyme Reagent Powder × 2 vials Powder × 10 vials -20℃, 12 months
Reagent 5 10 mmol/L 2-DG 1.5 mL × 1 vial 8 mL × 1 vial -20℃, 12 months
Reagent 6 0.3 nmol/μL Standard 2 mL × 1 vial 5 mL × 2 vials -20℃, 12 months
Reagent 7 KRPH Buffer Solution 55 mL × 1 vial 60 mL × 5 vials -20℃, 12 months
Black Microplate 96 wells No requirement
Plate Sealer 2 pieces


Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.

 

    Technical Data:

    Standard Curve:

    As the fluorescence value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:

    Concentration (nmol/L) 0 0.06 0.12 0.15 0.18 0.21 0.24 0.3
    Fluorescence value 5176 8677 11778 13332 14730 16049 17242 19704
    5211 8643 11635 13276 14717 15968 17037 19603
    Average fluorescence value 5194 8660 11707 13304 14724 16009 17140 19654
    Absoluted fluorescence value 0 3467 6513 8111 9530 10815 11946 14460