Flag-Tag Monoclonal Antibody Host Mouse
SKU: E-AB-48025-200
Flag-Tag Monoclonal Antibody Host Mouse
| SKU # | E-AB-48025 |
| Reactivity | All |
| Host | Mouse |
| Applications | WB, IF, IP, FCM |
Product Details
| Isotype | IgG2a |
| Host | Mouse |
| Reactivity | All |
| Applications | WB, IF, IP, FCM |
| Clonality | Monoclonal |
| Immunogen | Synthetic peptide corresponding to Flag tag conjugated to keyhole limpet haemocyanin. |
| Abbre | Flag-Tag |
| Synonyms | DDDDK epitope tag, DYKDDDDK epitope tag |
| Calculated MW | 45 kDa |
| Observed MW | 45 kDa |
| Concentration | 1 mg/mL |
| Buffer | PBS, pH 7.2. Contains 0.05% proclin 300. |
| Purification Method | Protein A/G Purification |
| Clone No. | 3H9 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:5000-10000 |
| IF | 1:500-2000 |
| IP | 3μg/sample |
| FCM | 0.2μg |
Background
FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. This antibody detects FLAG tag in most applications.