FITC-11-dUTP
FITC-11-dUTP
Introduction
FITC-11-dUTP is a modified triphosphate that can be linked to the 3 '-OH terminal of DNA by terminal transferase. This triphosphate is linked between fluorescein and the nitrogenous base by an 11-atom chain, which effectively prevents static quenching of fluorescence and improves the efficiency of nucleotide binding to the 3 '-OH terminal of DNA.
Product Details
Properties
| Application | DNA Fragmentation |
| Detection method | Fluorometric Method |
| Sample type | Paraffin section, frozen section, cell slide |
| Detection instrument | Fluorescence Microscope |
| Dye Type | FITC |
| Ex/Em (nm) | 490/520 |
| Channel set | FITC |
| Filter set | FITC |
| Storage | -20°C, shading light |
| Shipping | Ice bag |
| Expiration date | 24 months |
Detection Principle
Chromosome DNA breakage is an important marker event in cell apoptosis. A series of DNA 3 '-OH terminus is produced by DNA double-strand breaks in apoptotic cells or whenever there is a gap in one strand. The fluorescein labeled dUTP can be linked to the 3' -OH terminus of the broken DNA under the action of Terminal Deoxynucleotidyl Transferase (TdT). Fluorescence of dUTP conjugate can be detected by flow cytometry or fluorescence microscope.
Detection
Storage
Store in the dark at -20°C for 12 months. Avoid repeated freezing and thawing. It is not recommended to vortex.
Notes
- This kit is for research use only.
- For your safety and health, please wear the lab coat and disposable gloves before the experiments.