DUT Polyclonal Antibody Reactivity Human
SKU: E-AB-52896-200
DUT Polyclonal Antibody Reactivity Human
| SKU # | E-AB-52896 |
| Reactivity | Human, Rat |
| Host | Rabbit |
| Applications | IHC |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Rat |
| Applications | IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human DUT |
| Abbre | DUT |
| Synonyms | DUT, Deoxyuridine 5' triphosphate nucleotidohydrolase, Deoxyuridine 5'-triphosphate nucleotidohydrolase, Deoxyuridine triphosphatase, FLJ20622, dUTP nucleotidohydrolase, dUTP pyrophosphatase, dUTPase, dut, mitochondrial, nuclear isoform |
| Swissprot | |
| Cellular Localization | Mitochondrion and Nucleus. |
| Concentration | 1.08 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Epigenetics and Nuclear Signaling |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| IHC | 1:50-1:300 |
Background
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.