Bradford Protein Colorimetric Assay Kit

$ 170⁹⁵

Bradford Protein Colorimetric Assay Kit

SKU #	E-BC-K168-S
Detection Instrument	Spectrophotometer (595 nm)
Detection method	Colorimetric method

Product Details

Properties

Synonyms	TP
Sample Type	Serum, plasma, animal tissue
Sensitivity	0.026 mg/mL
Detection Range	0.026-1.2 mg/mL
Detection Method	Colorimetric method
Assay type	Quantitative
Assay time	50 min
Precision	Average inter-assay CV: 2.300% \| Average intra-assay CV: 1.800%
Other instruments required	Micropipettor, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	Reagent 1: 2-8℃, Reagent 2: RT
Valid period	12 months

Dilution of Sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.026-1.2 mg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	40-60
10% Mouse liver tissue homogenization	8-12
Rat serum	40-60

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Detection Principle

Coomassie brilliant blue G-250 is red under the free state, and it has the maximum absorbance at 465 nm. When the Coomassie brilliant blue G-250 combined to protein, the compound will have the maximum at 595 nm. The absorbance value is directly proportional to the protein content, so the concentration of total protein can be calculated directly by measuring the OD value at 595 nm.

Kit Components & Storage

Item	Component	Specification	Storage
Reagent 1	Chromogenic Agent Stock Solution	35 mL × 2 vials	2-8℃, shading light, 12 months
Reagent 2	0.563 mg BSA Standard	0.563 mg × 2 vials	RT, 12 months

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other.