Anti-MERS-CoV Nucleocapsid Monoclonal Antibody
SKU: E-AB-V1281-100
Anti-MERS-CoV Nucleocapsid Monoclonal Antibody
| SKU # | E-AB-V1281 |
| Reactivity | MERS-CoV |
| Host | Mouse |
| Applications | WB, ELISA |
Product Details
| Isotype | IgG1 |
| Host | Mouse |
| Reactivity | MERS-CoV |
| Applications | WB, ELISA |
| Clonality | Monoclonal |
| Immunogen | Recombinant MERS-CoV Nucleoprotein / NP protein (His Tag) |
| Synonyms | NP, Nucleocapsid, Nucleoprotein, Coronavirus NP, Coronavirus Nucleocapsid, Coronavirus Nucleoprotein, Cov np, Ncov NP, Novel Coronavirus Nucleoprotein |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS. |
| Purification Method | Protein A Affinity |
| Clone No. | 10 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:1000-1:5000 |
Background
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.