Anti-His(HHHHHH) Affinity Agarose, 2mL
SKU: EA-IP-008
Anti-His(HHHHHH) Affinity Agarose, 2mL
Background
Anti-His (HHHHHH) Affinity Agarose, made of high-quality His-tagged antibody covalently conjugated to agarose gel, has the characteristics of high loading capacity, high specificity, stable properties, are reusable, and can be used for immunoprecipitation related experiments such as affinity purification, immunoprecipitation (IP), and co-immunoprecipitation (Co-IP).
Performance Index
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Scope of application |
Affinity purification and immuno(co)precipitation of His-tagged fusion secreted proteins. His tag can be located at the N-terminus, C-terminus, or middle of the protein, such as the N-terminus His fusion protein (His-Protein), C-terminus His fusion protein (Protein-His), and Met modified intermediate His fusion protein (Met-His-Protein). Can only be used for affinity purification of secreted proteins.
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Antibody properties
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His-Tag Mouse mAb：Mouse IgG.
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Gel Properties
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Agarose gel granules, average size 100 μm.
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Binding capacity
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1 mL of Sepharose 4B agarose particles, covalently conjugated to 6 mg of mouse-derived IgG. 1 mL affinity gel can purify or precipitate at least 1.2 mg of His fusion protein.
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Liquid storag- e composition |
PBS containing preservatives and 50% glycerol. |
Storage
-20°C, 12 months.