AMPK alpha1/2 Polyclonal Antibody
SKU: E-AB-30491-200
AMPK alpha1/2 Polyclonal Antibody
| SKU # | E-AB-30491 |
| Reactivity | Human, Mouse, Rat, Monkey |
| Host | Rabbit |
| Applications | WB, IHC-p |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat, Monkey |
| Applications | WB, IHC-p |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from human AMPKα1/2 around the non-phosphorylation site of Thr183/172. |
| Abbre | AMPKα1/2 |
| Synonyms | 5'-AMP-activated protein kinase catalytic subunit alpha-1, ACACA kinase, AMPK, AMPK subunit alpha-1, AMPK1, Acetyl-CoA carboxylase kinase, HMGCR kinase, Hydroxymethylglutaryl-CoA reductase kinase, PRKAA1, PRKAA2, Tau-protein kinase PRKAA1 |
| Swissprot | |
| Calculated MW | 62 kDa |
| Observed MW | 63 kDa |
| Cellular Localization | Cytosol, Nucleus, nuclear speck, nucleoplasm, nucleus, Plasma Membrane, apical plasma membrane, Other locations: cytoplasm, intracellular, nucleotide-activated protein kinase complex. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cardiovascular, Metabolism, Neuroscience, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-1:2000 |
| IHC | 1:100-1:300 |
Background
AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively.