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Pyruvate is a central molecule in metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders.
Tribioscience’s Pyruvate Fluorometric Assay kit is designed to be a robust, simple, and high throughput method in which pyruvate is oxidized to generate hydrogen peroxide. The resulting hydrogen peroxide is catalyzed and reacts with the probe measured by the fluorometric method at Ex/Em=530/590 nm.
Assay Principle
Applications
Direct Assays: Detect as low as 1 µM of pyruvate in cells, serum, and other biological samples.
Storage and Handling
Store kit at -20°C. Shelf life of 12 months.