This enzyme is useful for enzymatic determination of L-malate and of glutamate oxaloacetate transaminase (GOT) in clinical analysis.
Tribioscience Malate dehydrogenase, Enzyme Activity (TBP0035)
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Tribioscience Malate dehydrogenase, Enzyme Activity (TBP0035)
Research Use Only. Not intended for human use.
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Preparation and Specification
Appearance: Slightly yellowish amorphous powder, lyophilized
Activity: GradeⅡ 40U/mg-solid or more
Contaminants: Glutamate oxaloacetate transaminase ≤1.0×10⁻³%
Lactate dehydrogenase ≤1.0×10⁻³%
NADH oxidase≤1.0×10⁻³%
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx. 140,000
Isoelectric point: pH 4.8±0.1
Michaelis constants: 5.4×10⁻⁵M (L-Malate), 5.0×10⁻⁶M (Oxaloacetate), 8.1×10⁻⁶M (NADH)
Structure: 4 subunits per enzyme molecule
Inhibitors: Hg⁺⁺
Optimum pH: 8.0
Optimum temperature: 70°C
pH Stability: pH 3.0-9.0 (25°C, 20hr)
Thermal stability: below 70°C (pH 7.5, 15min)
Applications