Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic index for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate bone disease.
Tribioscience’s Acid Phosphatase Colorimetric Assay Kit provides a high-sensitive, simple, and direct assay approach to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (λmax = 405 nm) when dephosphorylated by AP. The kit can detect as low as 20 µU acid phosphatase activity in samples.
Applications
Direct Assays: Acid phosphatase in serum, plasma, urine, and other bio-samples.
Key Features
- Flexible: Suitable for colorimetric assay.
- Accurate: Use 50 µL samples. Detection ranges from 0.4-200 µU in a 96-well plate for colorimetric assay.
- Simple and high-throughput: Just load-incubate-Read. The kit can be used for a robust method.
- Time-saving: less than 30 minutes.
For Research Use only
Standard Curve Preparations
