HIMEDIA TC-100 Insect Medium w/ L-Glutamine w/o Sodium Bicarbonate

HIMEDIA TC-100 Insect Medium w/ L-Glutamine w/o 10L

Product Description:

TC-100 is a modification done by Dr.Gardiner and Dr.Stockdale of the original formulation of Grace's insect medium. Sucrose, fructose and several Kreb's cycle intermediates were omitted from the original formula. The modification was done to optimise the production of Autographia californica NPV virions by cells from the fall armyworm, Spodoptera frugiperda. IM007 is TC-100 Insect Medium with L-glutamine. TC-100 is a fully defined medium and does not require supplementation with insect hemolymph. Instead, tryptose broth and fetal bovine serum provide the necessary growth factors . This medium supports the growth of several lepidopteran cell lines. Users are advised to review the literature for recommendations and physiological growth requirements for different cell lines.

Composition:

Directions:

1. Suspend 20.0gms in 900ml tissue culture grade water with constant, gentle stirring until the powder is completely dissolved. Do not heat the water.
2. Add 0.35gms sodium bicarbonate powder (TC230) or 4.7ml of 7.5% of sodium bicarbonate solution (TCL013) for each litre of medium. Stir until dissolved.
3. Adjust the pH to 6.2 using 1N KOH. Use of NaOH may cause precipitaion.
4. Make up the final volume to 1000ml.
5. Adjust the Osmolality as desired. For Lepidopterans cell line, osmolality of 340 - 360mOsm/KgH2O is recommended. The osmolality can be increased by 10mOsm/KgH2O by adding 0.4gms of potassium chloride (TC010) or 0.3gms of sodium chloride (TC046) to each litre of the medium Osmolality can be decreased by 10mOsm/KgH2O by adding 27.8ml of water to per litre of medium.
   
6. Sterilize the medium using a membrane filter with porosity of 0.22microns or less.
   
7. Aseptically dispense the medium in sterile containers.
8. Store liquid medium at and in dark till use.